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Microfluidics allows to simulate the strict selection process that occurs in the female tract to improve the reproduction outcome and success rate of artificial inseminations.
At Agrofluidics, we pioneer the commercial use of rheotaxis under different flow rates to evaluate the functionality of seminal samples. We are also advancing the development of thermotaxis and chemotaxis analyses, with the goal of delivering a fast, cost-effective, and innovative functional assessment of seminal quality.
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Assessment & Analysis
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Microfluidic analysis
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Innovation Pipeline
industry development pipeline
Current R&D Projects
SORTYX
Grupos Operativos Suprautonomicos
SortYX is an innovative project focused on developing a fast, automated, and non-invasive microfluidic system for sex-based sperm selection in pigs, with the aim of obtaining offspring of the desired sex and improving reproductive efficiency in the swine sector. The proposed solution seeks to overcome the limitations of current techniques, such as flow cytometry, through a microfluidic chip capable of separating X- and Y-bearing sperm cells without damaging them and with strong potential for implementation in artificial insemination centres. By combining reproductive biotechnology, microfluidics, computational simulation, and biological validation, the project aims to deliver a high-impact tool for genetic improvement, high-value extensive production, and a more sustainable and efficient pig farming industry.
Frequently asked questions about microfluidics applied to animal reproduction
What is microfluidics in animal reproduction?
Microfluidics is a technology that enables the manipulation of biological fluids within devices containing microscopic channels, using very small volumes under highly controlled conditions. In animal reproduction, its purpose is to more accurately replicate the physiological environment in which gametes and embryos develop, helping improve the quality, efficiency, and reproducibility of reproductive processes.
How is microfluidics used in animal reproduction?
Microfluidics is used to optimize different stages of the reproductive process, including sperm selection, in vitro fertilization, embryo culture and monitoring, and the development of more precise and less invasive systems for handling gametes and embryos. One of its most established applications is sperm selection, where it can help recover highly motile sperm cells with superior functional characteristics while reducing the cellular stress associated with some conventional techniques.
What is sperm rheotaxis and how is it used in semen evaluation?
Sperm rheotaxis is the ability of sperm cells to orient themselves and swim against a fluid flow. This behavior plays an important role in helping sperm progress through the female reproductive tract. Using microfluidic systems, these flow conditions can be recreated within microchannels to evaluate which sperm cells respond most effectively. Rheotaxis therefore becomes a functional tool for analyzing and selecting sperm with superior motility and quality characteristics.
Agrofluidics is a pioneer in the commercial application of rheotaxis under different flow rates to assess the functionality of semen samples.
What advantages does microfluidics offer over traditional semen analysis methods?
Microfluidics allows semen to be evaluated under conditions that closely resemble the physiological environment encountered by sperm within the female reproductive tract. Compared with traditional methods, it provides a more functional assessment by evaluating not only sperm concentration and motility, but also how sperm respond to physical stimuli such as fluid flow. In addition, microfluidic systems can reduce sample handling, reagent use, and cellular stress associated with conventional procedures that may negatively affect sample quality.
How does microfluidics contribute to more sustainable livestock production?
Agrofluidics contributes to more sustainable animal reproduction through technologies that improve the evaluation and selection of semen doses before artificial insemination. By identifying samples with superior functional quality, reproductive efficiency can be improved while reducing failures associated with lower-quality semen doses.
More efficient reproduction means better use of resources, including fewer repeat inseminations, reduced consumption of biological materials and reagents, and more precise reproductive planning. In addition, microfluidic systems operate with smaller sample volumes and less invasive processes for sperm cells. Technologies such as sperm sex selection may also contribute to more efficient and sustainable livestock production by better aligning output with production goals and reducing inefficiencies associated with undesired sex ratios.
How does Agrofluidics technology help reduce antibiotic use in swine reproduction?
In swine artificial insemination, antibiotics are commonly added to semen extenders to control bacterial growth during dose storage. However, reducing antibiotic use is becoming increasingly important in order to support sustainable reproduction practices and address the global challenge of antimicrobial resistance.
Agrofluidics addresses this challenge through microfluidic systems capable of processing semen doses using controlled physical mechanisms that help improve both sanitary and functional quality while reducing dependence on routine antibiotic use.
The objective is not to replace established hygiene and microbiological control practices, but rather to provide an additional technological tool that supports the production of cleaner, safer semen doses with lower reliance on antimicrobial treatments.